A defined co-culture method that enables the efficient in vitro growth of muscle, fat, and skin cells without serum or scaffolds. It improves biomass yield and tissue structure using optimized cell ratios and biological substrates. This system is ideal for scaling cultivated meat and leather production.
Cultivated meat and alternative leather producers seeking scalable, ethical, and cost-effective cell growth systems.
Serum-free or reduced-serum environments; optional use of biological substrates and macromolecular crowding agents.
• Reduces cost by eliminating fetal bovine serum • Enables scaffold-free tissue production • Improves total biomass yield • Suitable for structured products like steak, skin, or hide • Enhances growth conditions using natural cell ratios
• Uses precise co-culture ratios (e.g. 95:5) of muscle or fibroblast to fat cells for optimal cell proliferation and tissue formation • Supports growth on fibroblast-based skin substrates • Compatible with macromolecular crowding agents for enhanced performance • Applicable to both monolayers and 3D aggregates • Avoids synthetic scaffolds and animal-derived supplements
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