Serum-Free Co-Culture Method

Serum-Free Co-Culture Method

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Company
3D Bio-tissues3D Bio-tissues
Technology Pitch

A defined co-culture method that enables the efficient in vitro growth of muscle, fat, and skin cells without serum or scaffolds. It improves biomass yield and tissue structure using optimized cell ratios and biological substrates. This system is ideal for scaling cultivated meat and leather production.

Potential Users

Cultivated meat and alternative leather producers seeking scalable, ethical, and cost-effective cell growth systems.

Application Conditions

Serum-free or reduced-serum environments; optional use of biological substrates and macromolecular crowding agents.

Key Benefits

• Reduces cost by eliminating fetal bovine serum • Enables scaffold-free tissue production • Improves total biomass yield • Suitable for structured products like steak, skin, or hide • Enhances growth conditions using natural cell ratios

Unique Features

• Uses precise co-culture ratios (e.g. 95:5) of muscle or fibroblast to fat cells for optimal cell proliferation and tissue formation • Supports growth on fibroblast-based skin substrates • Compatible with macromolecular crowding agents for enhanced performance • Applicable to both monolayers and 3D aggregates • Avoids synthetic scaffolds and animal-derived supplements

Featured
Active
Problem Solved
Serum dependencyHigh media cost
Publication Date
April 24, 2025
Tech Category
Co-culture & Structured Tissue Fabrication
patent

WO2025083418A2

Contact

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